Hi, everyone! I'm Martin. I'm a third-year student at Georgetown majoring in Biology (with a concentration in Molecular Bio), and I'm planning to minor in Japanese. My focus has always been on cancer biology; so many people I've known have been affected by it, and my life's goal is to contribute to a cure. I'm half-American, half-Bulgarian, the child of two diplomats; I speak English fluently, and intermediate Bulgarian, Japanese, and Spanish. I have a lot of different interests outside of science: basketball and baseball, chess, politics, music, and a whole other list that's way too long for this blurb. Here's a random fact about me which I think is kinda cool: I once saw the tallest manmade thing on Earth (the Burj Khalifa) and the tallest thing on Earth, period (Mt. Everest) from the window of the same flight!
My research project for Laidlaw this summer is an application of the fields I've spent so much time studying- the focus is on cancer genetics, molecular biology, and laboratory technique. The goal of this project is to uncover information about a certain protein called SON, which is thought to be involved in the processes of gene splicing and transcription into RNA. Errors in these functions often result in cancer, so figuring out the roles and behaviors of proteins like SON could really help future endeavors like genetic libraries and drug discovery programs. I won't bog this down with an excessively detailed explanation of my method, but in brief, I'm using CRISPR technology to edit the SON gene and add DNA which encodes something called a protein tag. CRISPR can use a few different DNA repair techniques as a sort of "glue" to insert this protein tag; I'll be testing two of these techniques (NHEJ and MMEJ) to add depth to the experimental process. When the modified gene gets translated into SON protein, this tag gets produced as well. The tag can be lit up and investigated with a fluorescent microscope to determine where in the cell the SON protein localizes; it can also be targeted for degradation, allowing me to research the behaviors of cells with no SON protein and thus deduce its function through this knockout treatment. Which of these goals I can accomplish depends on the time and resources I have available, but I hope that summary sheds light on the problems I'm trying to tackle with this research project. In a word, I want to find out what SON does, and I'm going to use CRISPR-mediated protein tags to find out.
