Migraines affect roughly one third of the population, yet the exact mechanisms by which they develop are not fully understood. Rodent models are often used to study neurological and pain-related conditions such as migraines. However, mice do not naturally develop migraines which poses challenges in identifying, studying, and quantifying a headache presence and pain response. The goal of this project is to develop a robust protocol to trigger a migraine-like headache in a mouse model and quantify their behavioral response to better understand migraine pathophysiology. The mice in this study first underwent a 5 day period of chronic sensory innervation of the meninges (the nerves whose activation is considered to be responsible for migraines) using an optogenetic approach to establish a baseline migraine-like condition. They were then injected with 0.1 mg, 1 mg, and 10 mg of nitroglycerin, a drug that triggers migraines in humans, over the course of 9 days. The level of squinting of the animals in the dark as well as with ambient light in response to the drug was recorded and is predicted to correlate with the level of pain and light hypersensitivity (photophobia) they experience. The predicted outcome is a reliable protocol to measure migraine-like pain within a mouse. The results from this study will inform future investigation into migraine therapeutic treatments and pathophysiology.